Image reversal reactive immersion lithography improves the detection limit of focal molography

26. November 2018

Frutiger A. et al. Opt. Lett. 43, 5801-5804 (2018)

Focal molography is a label-free optical biosensing method that relies on a coherent pattern of binding sites for biomolecular interaction analysis. Reactive immersion lithography (RIL) is central to the patterning of molographic chips but has potential for improvements. Here, we show that applying the idea of image reversal to RIL enables the fabrication of coherent binding patterns of increased quality (i.e., higher analyte efficiency). Thereby the detection limit of focal molography in bi ological assays can be improved.