Literature on Focal Molography

High-throughput thermodynamic fingerprinting of protein–ligand interactions by DNA-directed focal molography

Oehninger, Notova, Frutiger

Oehninger, J., Notova, S., & Frutiger, A. (2026). PLOS One, 21(10), e0354008. https://doi.org/10.1371/journal.pone.0354008

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Same target, different stickiness: polyreactivity of therapeutic antibodies

lino Biotech AG

Six clinical antibodies, including two same-target pairs, ranked by polyreactivity against an eight-ligand panel on one focal molography chip. Briakinumab scored 69-fold above ...

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Parallel kinetic validation of DNA-encoded library hits

lino Biotech AG

Twenty-four on-DNA hits from a DEL selection, validated in parallel against CAIX and the antitarget CAII on one focal molography chip, without purification. Four sub-nanomolar ...

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Discovery on Target 2026: From Living-Cell GPCRs to On-DNA DEL Hits

Dr. Volker Gatterdam

Market Development Manager·lino Biotech AG

Label-free kinetics on GPCRs in living cells and on-DNA validation of DEL hits, measured in crude matrices without purification. Recorded at Discovery on Target 2026 in Boston.

Development of an Fc-Capturing Protein for High-Throughput Screening at Miltenyi Biotec

Artem Siemens

Team Coordinator, Binder Discovery Group·Miltenyi Biotec

Recorded at the 3rd focal Molography User Meeting, Artem Siemens (Miltenyi Biotec) shows how a homodimeric capture nanobody, immobilized by DNA-directed immobilization (DDI), ...

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Focal molography for the Fc receptor profiling of therapeutic anti-IgE antibodies

Dr. Paul Engeroff

University of Bern

A multiplex Fc receptor panel on focal molography maps where omalizumab, ligalizumab and their immune complexes with IgE and allergen bind, across all human Fc gamma and IgE ...

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Focal Molography Enables Rapid Evaluation of Miniprotein Plasma Protein Binding

Alyssa Anderson

Head of Protein Characterization·AI Proteins

Plasma protein binding of 15 designed miniproteins measured directly in plasma on one 64-plex chip, validated against ultracentrifugation and extended by kinetic information.

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Measuring the affinity of DNA-small molecule conjugates to target proteins, application to PROTACs

Prof. Andreas Brunschweiger

University of Würzburg

Focal molography measures how ligand, linker and oligonucleotide shape the target binding of DNA-small molecule conjugates, from DNA-conjugated PROTACs on the E3 ligase VHL to hit ...

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lino Update: New Reagents, Software Features and Developments

Sophia Zhai, Alexander Geiger, Lukas Heuberger

lino Biotech AG

New oligo adapters for DEL hit validation and for binders straight from lysate, four multiplex panels for antibody developability, and guided software workflows for the MACS ...

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Molography for integrin-mediated cancer cell adhesion

Szabolcs Novák

HUN-REN Centre for Energy Research

Focal molography follows how living HeLa cancer cells adhere to RGD-functionalized sensor chips and separates where integrins bind from how much mass arrives, revealing three ...

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On-DNA Kinetics at Philochem: What SPR Cannot Deliver

Nicholas Favalli

Group Leader, Chemistry R&D·Philochem AG, Zurich

Kinetic profiles for DEL hits that Biacore SPR and fluorescence polarization could not resolve, obtained directly on-DNA with focal molography.

DEL Hit Validation: The On-DNA Advantage

Joshua Alper, PhD

Principal Consultant·Alper Consulting, LLC

Why on-DNA binding confirmation matters at the pharma-portfolio level, and how focal molography enables validation in biologically relevant matrices.

Organization and Dynamics of Focal Adhesions: Light Diffraction Analysis of Cellular Adhesion on Nanopatterned Surfaces

Szekacs, I., Novák, S., Kovacs, B., Dicső, Z., Péter, B., Bonyár, A., Popov, R., Frutiger, A., & Horvath, R. (2026). ACS Applied Materials & Interfaces. ...

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High-Throughput Binding Kinetic Measurements of DNA-Encoded Library-Derived Hits by Focal Molography

Favalli, N., Velti, C., Campari, L., Heuberger, L., Fabbri, M., Müller, M., Puglioli, S., Cazzamalli, S., Neri, D., Frutiger, A., & Oehler, S. (2026). Journal of Medicinal ...

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Focal Molography in Complex Media

Prof. Dominik Meinel

Professor of Biophysics·FHNW Muttenz

In this live webinar, Prof. Dominik Meinel (FHNW) presents how focal molography enables label-free biomolecular interaction analysis in complex media. The examples include serum, ...

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High-Throughput Pharmacologic Activity Profiling of Antibodies

Poster presented at PEGS Boston 2026 (Booth 615, Poster A073). Demonstrates an 8-plex Fc receptor profiling panel, anti-CD20 serum protein binding kinetics, and on-DNA DEL hit ...

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Investigations into Linker Effects of DNA-VHL Ligand Conjugates by Multiplexed Affinity Measurements Using Focal Molography

Raschke, P., Notova, S., Gatterdam, V., Frutiger, A., & Brunschweiger, A. (2026). RSC Chemical Biology, Advance Article. https://doi.org/10.1039/D6CB00011H

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Screening 20 DNA-PROTAC Linkers in a Single Experiment

This scientific poster was presented at the "6th TPD & Induced Proximity Summit Europe" in London, UK (Mar 10-12, 2026) by Dr. Andreas Frutiger. It investigates linker effects ...

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MACS® Matchmaker - Biomolecular Interaction Analysis in Real Samples

This scientific poster was presented at the "PEGS Europe 2025" conference in Lisbon, PT (Nov 11-13, 2025). It describes four examples in which focal molography technology advances ...

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Anti-His-tag capture system: fast, reversible and convenient protein immobilization

This application note describes a straightforward protocol for immobilizing His-tagged proteins to the surface of biosensor chips used on the MACS® Matchmaker platform.

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Accelerating Drug Discovery with focal molography

This scientific poster was presented at the "ELRIG Drug Discovery 2025" meeting in Liverpool, UK (Oct 21-22, 2025). It describes label-free detection and analysis of biomolecular ...

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Exploring beta-catenin and TCF4 interaction in complex environments by means of novel biosensing platform focal molography

Philipp Cedro, Roman Popov, Maxime Karrer, Jean-Christophe Hau, Eric-André Kusznir, Ralf Thoma, Andreas Frutiger, Matthias Lauer, Sylwia Huber PLoS One 20(9):e0333554. ...

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On-DNA Hit Validation from DNA-Encoded Library Screenings by Focal Molography

This scientific poster was presented at the "13th International Symposium on DNA-Encoded Chemical Libraries" in Boston, MA, USA (Aug 14-15, 2025). It describes an on-DNA method ...

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FcRn Species Panel: cross-species binding kinetics on a single chip

This application note resolves cross-species FcRn binding kinetics for two IgG1 antibodies against six species (human, cynomolgus, marmoset, rat, minipig, mouse). All values come ...

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Novalix 2025: Biophysics in Drug Discovery

Dr. Andreas Frutiger

Head of R&D·lino Biotech AG

Fast and label-free kinetic characterization of up to 54 DEL hits in one go, on-DNA and without off-DNA synthesis.

Anti-Fc capture system: convenient and fast immobilization of antibodies

The document introduces an anti-Fc capture system for fast and convenient immobilization of antibodies on focal molography sensor chips. It is based on oligonucleotide-conjugated ...

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Focal Molography: A New Frontier in Biosensing

Focal molography was introduced in our last article as a technology that detects molecular interactions without picking up environmental noise. This article walks through its ...

An Introduction to Focal Molography

SPR offers many advantages in biosensing, but environmental noise limits it. Has any biosensor overcome this? Focal molography detects molecular interactions without picking up ...

MACS® Matchmaker Instrument Brochure

Comprehensive overview of the MACS® Matchmaker label-free biosensor platform, including kinetic characterization modes (SCK, MCK), concentration determination, integrated ...

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The Big Con of SPR, and How to Solve it

Why surface plasmon resonance, like all refractometric biosensors, suffers from environmental noise and needs very high precision, and how Fourier space avoids that.

Surface Plasmon Resonance (SPR)

Surface plasmon resonance (SPR) is a widely used label-free assay for detecting molecular interactions. This article explains what it is used for, how it works, and its main ...

How scientists use MACS Matchmaker

Multiple researchers

Researchers explain how focal molography delivers sensitivity, robustness and kinetic precision in real biological samples.

Heterogeneous vs. Homogeneous Assays

What are the similarities between these assays and how do they differ? When should one favor one over the other? Discover more about practical examples of heterogeneous and ...

Practical Examples of Biosensors

What is a biosensor used for? Read more about following two practical examples of label biosensors: the pregnancy test and the glucose meter.

What is a biosensor?

What is a Biosensor? Read more about the Definition of a Biosensor, Basic Elements and the Classification between Label vs. Label-Free Biosensors.

Focal Molography Allows for Affinity and Concentration Measurements of Proteins in Complex Matrices with High Accuracy

Dirscherl, L.; Merz, L.S.; Kobras, R.; Spies, P.; Frutiger, A.; Gatterdam, V.; Meinel, D.M. Focal Molography Allows for Affinity and Concentration Measurements of Proteins in ...

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Anti-biotin capture system: fast and easy solution for the immobilization and characterization of your biotinylated ligands

The document presents the development of an anti-biotin capture system for focal Molography to enable straightforward immobilization and kinetic characterization of biotinylated ...

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Fast and convenient hit validation for DNA-encoded libraries

The document describes how focal molography works with DNA-encoded library (DEL) technology to simplify screening and validation of small molecule hits in drug discovery. The ...

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Context Matters - Real Samples, Real Results with Label-free Technology

The experiments highlighted the method’s reliability in differentiating molecular interactions in both PBS-T buffer and human serum. Using anti-GFP VHH, the technique demonstrated ...

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Characterizing small molecule hits from DNA-encoded library screenings by focal Molography

The document shows how focal molography works with DNA-encoded library (DEL) technology for efficient screening and kinetic characterization of small molecules. This approach ...

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Total internal reflection focal molography (TIR-M)

Blickenstorfer, Y., Borghi, L., Reichmuth, A. M., Fattinger, C., Vörös, J., & Frutiger, A. (2021). Sensors and Actuators B: Chemical, 349, 130746.

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Quantitative Diffractometric Biosensing

Blickenstorfer, Y., Müller, M., Dreyfus, R., Reichmuth, A. M., Fattinger, C., & Frutiger, A. (2021). Physical Review Applied, 15(3), 034023.

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Investigating complex samples with molograms of low-affinity binders

Reichmuth, A. M., Kübrich, K., Blickenstorfer, Y., Frutiger, A., Momotenko, D., Gatterdam, V., ... & Vörös, J. (2021). ACS sensors, 6(3), 1067-1076.

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Ultra-Stable Molecular Sensors by Sub-Micron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part I. The Concept of a Spatial Affinity Lock-in Amplifier

Frutiger, A., Fattinger, C., & Vörös, J. (2021). Sensors, 21(2), 469.

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Ultra Stable Molecular Sensors by Submicron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part II. Experimental Demonstration

Frutiger, A., Gatterdam, K., Blickenstorfer, Y., Reichmuth, A. M., Fattinger, C., & Vörös, J. (2020). Sensors, 21(1), 9.

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Quantification of Molecular Interactions in Living Cells in Real Time using a Membrane Protein Nanopattern

Reichmuth, A. M., Zimmermann, M., Wilhelm, F., Frutiger, A., Blickenstorfer, Y., Fattinger, C., ... & Vörös, J. (2020). Analytical chemistry, 92(13), 8983-8991.

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