Resources on focal Molography

This scientific poster, presented at the "PEGS Europe 2025" conference in Lisbon, PT (Nov 11-23, 2025),  describes four different examples in which focal molography technology advances protein research, including characterization of VHH antibodies, analysis of "difficult proteins" or even cell-based interaction analysis. 

This application note describes a straightforward protocol for immobilizing His-tagged proteins to the surface of biosensor chips used on the MACS® Matchmaker platform.

This scientific poster, presented at the "ELRIG Drug Discovery 2025" meeting in Liverpool, UK (Oct 21-22, 2025),  describes an innovative solution for the label-free detection and analysis of biomolecular interactions, offering high sensitivity, specificity, and robustness.

Philipp Cedro, Roman Popov, Maxime Karrer, Jean-Christophe Hau, Eric-André Kusznir, Ralf Thoma, Andreas Frutiger, Matthias Lauer, Sylwia Huber PLoS One 20(9):e0333554. https://doi.org/10.1371/journal.pone.0333554

This scientific poster, presented at the "13th International Symposium on DNA-Encoded Chemical Libraries" in Boston, MA, USA (Aug 14-15, 2025),  describes a novel hit characterization methodology in drug discovery, for kinetic and affinity assessments after DEL screenings.

Dirscherl, L.; Merz, L.S.; Kobras, R.; Spies, P.; Frutiger, A.; Gatterdam, V.; Meinel, D.M. Focal Molography Allows for Affinity and Concentration Measurements of Proteins in Complex Matrices with High Accuracy. Biosensors 2025, 15, 66. https://doi.org/10.3390/bios15020066

The experiments highlighted the method’s reliability in differentiating molecular interactions in both PBS-T buffer and human serum. Using anti-GFP VHH, the technique demonstrated comparable kinetic parameters and sensitivity. The robustness against non-specific effects and its compatibility with physiological conditions make focal molography valuable for biological and therapeutic research.

Teh document shows the integration of focal molography with DNA-encoded library (DEL) technology, streamlining drug discovery by enabling efficient screening and kinetic characterization of small molecules. This approach accelerates the identification of potential drug candidates, offering a high-throughput, robust platform for studying molecular interactions and advancing therapeutic development.

Blickenstorfer, Y., Borghi, L., Reichmuth, A. M., Fattinger, C., Vörös, J., & Frutiger, A. (2021). Sensors and Actuators B: Chemical, 349, 130746.

Blickenstorfer, Y., Müller, M., Dreyfus, R., Reichmuth, A. M., Fattinger, C., & Frutiger, A. (2021). Physical Review Applied, 15(3), 034023.

Frutiger, A., Fattinger, C., & Vörös, J. (2021). Sensors, 21(2), 469.

Frutiger, A., Gatterdam, K., Blickenstorfer, Y., Reichmuth, A. M., Fattinger, C., & Vörös, J. (2020). Sensors, 21(1), 9.

Reichmuth, A. M., Zimmermann, M., Wilhelm, F., Frutiger, A., Blickenstorfer, Y., Fattinger, C., ... & Vörös, J. (2020). Analytical chemistry, 92(13), 8983-8991.