Anti-His-tag capture system: fast, reversible and convenient protein immobilization

The His-tag (histidine tag) is a short sequence that is genetically fused to a target protein. It typically comprises six to twelve histidine residues (His₆ to His₁₂). The tag is widely employed in protein research and biomanufacturing because it simplifies purification, immobilization, and detection.

Here, we present a simple, fast, and reliable method for immobilizing His-tagged proteins via the Oligo Adapter trisNTA capture system. This approach is based on an oligonucleotide-conjugated trisNTA chelator head, which anchors the His-tagged ligand to the sensor chip surface.