01 / EXPRESS
Start with lysate
Express each AI-designed binder separately with a SpyTag or Twin-Strep-tag. Retain its design ID throughout the workflow.
High-throughput screening for AI-designed binders
AI designs binders.
Screen them at scale.
Measure up to 64 AI-designed binders in parallel on one sensor chip. Go from crude expression to binding kinetics with MACS® Matchmaker.
64-plex kinetics · Directly from lysate
Explore the workflowDiscuss your panelUp to 64 in parallelBinder kinetics on one sensor chip
96-well workflowOrganize coded pools for sequential screening
Feedback for AI designUse measured binding to prioritize the next round
Experimental feedback for AI protein design
Turn design libraries
into measured priorities.
AI protein design generates candidates for experimental testing. High-throughput binding characterization helps you compare those designs and select the next round.
MACS Matchmaker measures up to 64 binders in parallel, directly from lysate. Link each kinetic result to its design ID to guide selection and subsequent model refinement.
01 / COMPUTE
Design a library
Generate and prioritize candidate sequences in your AI design workflow.
02 / EXPERIMENT
Screen 64 in parallel
Measure binding kinetics across coded panels of expressed designs.
03 / LEARN
Inform the next round
Return binding profiles to your design process and select the next candidates.
Measured binding → candidate selection → next AI design round ↻
High-throughput experimental screening
One pool. Up to 64 binder profiles.
Express your AI-designed candidates, assign DNA codes and pool up to 64 binders. DNA-directed immobilization sorts each binder onto its assigned sensor spot.
02 / CODE
Assign a DNA code
Add a different coded capture adapter to each lysate, with binder in excess over adapter.
03 / POOL
Pool up to 64 binders
Combine coded lysates into one well. Organize additional pools across a 96-well plate for larger design libraries.
04 / MEASURE
Measure in parallel
Self-sort the pool on the chip, then measure target binding across up to 64 candidates in parallel. Regenerate and load the next pool.
Workflow schematic. The curve illustrates single-cycle kinetics; it is not experimental data.
Two capture routes
Choose the tag that fits your construct.
The capture adapter connects your tagged binder to its DNA code. Both routes feed into the same split-and-pool workflow.
Covalent capture
SpyTag + SpyCatcher
SpyTag binder→SpyCatcher+DNA code
SpyTag on the binder reacts with the Oligo Adapter SpyCatcher to form a covalent bond. The adapter's DNA code provides the address on the sensor chip.
Affinity capture
Twin-Strep-tag® + Strep-Tactin® XT
Twin-Strep binder→Strep-Tactin XT+DNA code
Twin-Strep-tag on the binder binds the Oligo Adapter Strep-Tactin® XT. This capture interaction attaches the binder to its DNA address.
Scale beyond a single panel.
Combine 64-plex measurements with a 96-well pooling workflow to plan larger AI design campaigns. Measure each pool sequentially against the target.
96×64=6,144
Nominal design capacity: 96 pools × 64 positions, before controls and replicates. Actual throughput depends on the assay and regeneration performance.
Data for the next design round
Rank AI designs by measured binding.
Target concentration series reveal how each interaction develops over time. Where the data support a kinetic model, compare three complementary parameters.
How fast does it bind?
Compare association rates to see how quickly each binder engages the target.
How long does it stay?
Compare dissociation rates to distinguish interactions with different complex lifetimes.
How strong is the interaction?
Combine association and dissociation rates to estimate affinity under an appropriate binding model.
After the binding screen
Binding is round one. Developability is round two.
A design that binds its target can still fail later through nonspecific binding or poor behavior in plasma. Take your shortlisted binders into developability panels that run on the same instrument, directly in complex samples.
Polyreactivity Panel
Flag nonspecific binding and other developability liabilities before a design moves forward.
Explore the Polyreactivity PanelPlasma Protein Binding
See how your lead designs behave in undiluted human plasma.
Explore Plasma Protein BindingDevelopability Data Service
Send your shortlist and receive a developability package run by our scientists.
Explore the servicePractical questions
Before you build your panel.
Do I need to purify every binder?
The workflow captures tagged binders directly from lysate. Tag accessibility, expression level and sample compatibility need to be checked for your constructs.
Is Strep-Tactin the tag on my binder?
No. Twin-Strep-tag is fused to the binder; Strep-Tactin XT is the capture adapter. In the other route, SpyTag is fused to the binder and SpyCatcher is the adapter.
Why use less adapter than binder?
Binder excess favors loading of the coded adapters before pooling. The suitable ratio depends on expression level and capture efficiency.
What does the DNA code identify?
The code assigns a binder to a complementary address on the sensor chip. It is an immobilization handle, not the binder’s protein sequence.
What does 64-plex mean for my screening campaign?
Up to 64 binders can be measured in parallel on one sensor chip. Controls and replicates reduce the number of unique candidates. A 96-well plate can organize multiple pools; measurement time and chip reuse depend on the assay.
How do the results support AI design?
Map each DNA code to its design ID, then use measured binding profiles to select candidates and inform your next design round. Model training and sequence generation remain part of your computational workflow.
Related
More from the same platform.
Encoded Library Screening
A related 64-plex workflow for small molecules: expand a hit into a library and measure every member.
Explore Encoded Library ScreeningOligo Adapters
DNA-coded adapters that load each ligand onto its assigned sensor spot by DNA-directed immobilization.
Explore the adaptersCustom Measurement Service
Send samples, receive kinetics. No instrument required.
Explore the service