Ultra-Stable Molecular Sensors by Sub-Micron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part I. The Concept of a Spatial Affinity Lock-in Amplifier

Development of an Fc-Capturing Protein for High-Throughput Screening at Miltenyi Biotec

Artem Siemens

Team Coordinator, Binder Discovery Group·Miltenyi Biotec

Capture and immobilization · Antibodies and Fc biology

Recorded at the 3rd focal Molography User Meeting, Artem Siemens (Miltenyi Biotec) shows how a homodimeric capture nanobody, immobilized by DNA-directed immobilization (DDI), ...

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On-DNA Kinetics at Philochem: What SPR Cannot Deliver

Nicholas Favalli

Group Leader, Chemistry R&D·Philochem AG, Zurich

DEL and hit validation

Kinetic profiles for DEL hits that Biacore SPR and fluorescence polarization could not resolve, obtained directly on-DNA with focal molography.

DEL Hit Validation: The On-DNA Advantage

Joshua Alper, PhD

Principal Consultant·Alper Consulting, LLC

DEL and hit validation

Why on-DNA binding confirmation matters at the pharma-portfolio level, and how focal molography enables validation in biologically relevant matrices.