About the talk
Dr. Andreas Frutiger, Head of R&D at lino Biotech AG, spoke at the 11th Novalix Biophysics in Drug Discovery Conference (June 2025). He presented how focal molography addresses the DEL hit validation bottleneck.
The DEL validation problem
The traditional DEL workflow requires off-DNA synthesis and characterization of every promising hit. This generates significant work in the chemistry department while producing limited biologically relevant information. The bottleneck scales poorly when a selection returns tens or hundreds of hits worth pursuing.
Focal molography's contribution
By exploiting the DNA tag for directed immobilization, focal molography enables direct on-DNA characterization. It delivers fast and label-free kinetic profiling of up to 54 DEL hits in a single instrument run, without the need for off-DNA synthesis. Kinetic parameters kon and koff are read directly from the on-DNA compound bound to the sensor surface.
About the speaker
Dr. Andreas Frutiger is Head of R&D at lino Biotech AG. He has co-authored the foundational publications on focal molography as a label-free biomolecular interaction analysis technology.
More resources
Oct 2026
High-throughput thermodynamic fingerprinting of protein–ligand interactions by DNA-directed focal molography
Oehninger, J., Notova, S., & Frutiger, A. (2026). PLOS One, 21(10), e0354008. https://doi.org/10.1371/journal.pone.0354008
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Oct 2026
Same target, different stickiness: polyreactivity of therapeutic antibodies
Six clinical antibodies, including two same-target pairs, ranked by polyreactivity against an eight-ligand panel on one focal molography chip. Briakinumab scored 69-fold above ...
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Oct 2026
One on-DNA ligand against many targets: selectivity profiling for DEL hit validation
On-DNA oncoFAP profiled against human, mouse and canine FAP and five unrelated proteins on one focal molography chip layout. Full kinetics on every species variant, flat responses ...
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