Documents on Focal Molography

High-throughput thermodynamic fingerprinting of protein–ligand interactions by DNA-directed focal molography

Oehninger, Notova, Frutiger

Oehninger, J., Notova, S., & Frutiger, A. (2026). PLOS One, 21(10), e0354008. https://doi.org/10.1371/journal.pone.0354008

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Same target, different stickiness: polyreactivity of therapeutic antibodies

lino Biotech AG

Six clinical antibodies, including two same-target pairs, ranked by polyreactivity against an eight-ligand panel on one focal molography chip. Briakinumab scored 69-fold above ...

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One on-DNA ligand against many targets: selectivity profiling for DEL hit validation

lino Biotech AG

On-DNA oncoFAP profiled against human, mouse and canine FAP and five unrelated proteins on one focal molography chip layout. Full kinetics on every species variant, flat responses ...

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Parallel kinetic validation of DNA-encoded library hits

lino Biotech AG

Twenty-four on-DNA hits from a DEL selection, validated in parallel against CAIX and the antitarget CAII on one focal molography chip. Four sub-nanomolar binders with ...

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Organization and Dynamics of Focal Adhesions: Light Diffraction Analysis of Cellular Adhesion on Nanopatterned Surfaces

Szekacs, I., Novák, S., Kovacs, B., Dicső, Z., Péter, B., Bonyár, A., Popov, R., Frutiger, A., & Horvath, R. (2026). ACS Applied Materials & Interfaces. ...

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High-Throughput Binding Kinetic Measurements of DNA-Encoded Library-Derived Hits by Focal Molography

Favalli, N., Velti, C., Campari, L., Heuberger, L., Fabbri, M., Müller, M., Puglioli, S., Cazzamalli, S., Neri, D., Frutiger, A., & Oehler, S. (2026). Journal of Medicinal ...

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High-Throughput Pharmacologic Activity Profiling of Antibodies

Poster presented at PEGS Boston 2026 (Booth 615, Poster A073). Demonstrates an 8-plex Fc receptor profiling panel, anti-CD20 serum protein binding kinetics, and on-DNA DEL hit ...

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Investigations into Linker Effects of DNA-VHL Ligand Conjugates by Multiplexed Affinity Measurements Using Focal Molography

Raschke, P., Notova, S., Gatterdam, V., Frutiger, A., & Brunschweiger, A. (2026). RSC Chemical Biology, Advance Article. https://doi.org/10.1039/D6CB00011H

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Screening 20 DNA-PROTAC Linkers in a Single Experiment

This scientific poster was presented at the "6th TPD & Induced Proximity Summit Europe" in London, UK (Mar 10-12, 2026) by Dr. Andreas Frutiger. It investigates linker effects ...

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MACS® Matchmaker - Biomolecular Interaction Analysis in Real Samples

This scientific poster was presented at the "PEGS Europe 2025" conference in Lisbon, PT (Nov 11-13, 2025). It describes four examples in which focal molography technology advances ...

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Anti-His-tag capture system: fast, reversible and convenient protein immobilization

This application note describes a straightforward protocol for immobilizing His-tagged proteins to the surface of biosensor chips used on the MACS® Matchmaker platform.

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Accelerating Drug Discovery with focal molography

This scientific poster was presented at the "ELRIG Drug Discovery 2025" meeting in Liverpool, UK (Oct 21-22, 2025). It describes label-free detection and analysis of biomolecular ...

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Exploring beta-catenin and TCF4 interaction in complex environments by means of novel biosensing platform focal molography

Philipp Cedro, Roman Popov, Maxime Karrer, Jean-Christophe Hau, Eric-André Kusznir, Ralf Thoma, Andreas Frutiger, Matthias Lauer, Sylwia Huber PLoS One 20(9):e0333554. ...

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On-DNA Hit Validation from DNA-Encoded Library Screenings by Focal Molography

This scientific poster was presented at the "13th International Symposium on DNA-Encoded Chemical Libraries" in Boston, MA, USA (Aug 14-15, 2025). It describes an on-DNA method ...

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FcRn Species Panel: cross-species binding kinetics on a single chip

This application note resolves cross-species FcRn binding kinetics for two IgG1 antibodies against six species (human, cynomolgus, marmoset, rat, minipig, mouse). All values come ...

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Anti-Fc capture system: convenient and fast immobilization of antibodies

The document introduces an anti-Fc capture system for fast and convenient immobilization of antibodies on focal molography sensor chips. It is based on oligonucleotide-conjugated ...

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MACS® Matchmaker Instrument Brochure

Comprehensive overview of the MACS® Matchmaker label-free biosensor platform, including kinetic characterization modes (SCK, MCK), concentration determination, integrated ...

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Focal Molography Allows for Affinity and Concentration Measurements of Proteins in Complex Matrices with High Accuracy

Dirscherl, L.; Merz, L.S.; Kobras, R.; Spies, P.; Frutiger, A.; Gatterdam, V.; Meinel, D.M. Focal Molography Allows for Affinity and Concentration Measurements of Proteins in ...

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Anti-biotin capture system: fast and easy solution for the immobilization and characterization of your biotinylated ligands

The document presents the development of an anti-biotin capture system for focal Molography to enable straightforward immobilization and kinetic characterization of biotinylated ...

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Fast and convenient hit validation for DNA-encoded libraries

The document describes how focal molography works with DNA-encoded library (DEL) technology to simplify screening and validation of small molecule hits in drug discovery. The ...

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Context Matters - Real Samples, Real Results with Label-free Technology

The experiments highlighted the method’s reliability in differentiating molecular interactions in both PBS-T buffer and human serum. Using anti-GFP VHH, the technique demonstrated ...

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Characterizing small molecule hits from DNA-encoded library screenings by focal molography

The document shows how focal molography works with DNA-encoded library (DEL) technology for efficient screening and kinetic characterization of small molecules. This approach ...

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Total internal reflection focal molography (TIR-M)

Blickenstorfer, Y., Borghi, L., Reichmuth, A. M., Fattinger, C., Vörös, J., & Frutiger, A. (2021). Sensors and Actuators B: Chemical, 349, 130746.

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Quantitative Diffractometric Biosensing

Blickenstorfer, Y., Müller, M., Dreyfus, R., Reichmuth, A. M., Fattinger, C., & Frutiger, A. (2021). Physical Review Applied, 15(3), 034023.

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Investigating complex samples with molograms of low-affinity binders

Reichmuth, A. M., Kübrich, K., Blickenstorfer, Y., Frutiger, A., Momotenko, D., Gatterdam, V., ... & Vörös, J. (2021). ACS sensors, 6(3), 1067-1076.

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Ultra-Stable Molecular Sensors by Sub-Micron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part I. The Concept of a Spatial Affinity Lock-in Amplifier

Frutiger, A., Fattinger, C., & Vörös, J. (2021). Sensors, 21(2), 469.

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Ultra Stable Molecular Sensors by Submicron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part II. Experimental Demonstration

Frutiger, A., Gatterdam, K., Blickenstorfer, Y., Reichmuth, A. M., Fattinger, C., & Vörös, J. (2020). Sensors, 21(1), 9.

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Quantification of Molecular Interactions in Living Cells in Real Time using a Membrane Protein Nanopattern

Reichmuth, A. M., Zimmermann, M., Wilhelm, F., Frutiger, A., Blickenstorfer, Y., Fattinger, C., ... & Vörös, J. (2020). Analytical chemistry, 92(13), 8983-8991.

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